human il 6 elisa kit  (R&D Systems)

Journal: Journal of Inflammation Research

Article Title: The Relationship Between Decreased Serum Klotho Protein and Cognitive Impairment in Patients After Abdominal Surgery

doi: 10.2147/JIR.S593405

Figure Lengend Snippet: The correlation between serum Klotho and clinical indicators. Pearson correlation test was performed between Klotho with ( A ) duration of anesthesia, ( B ) postoperative MoCA scores, ( C ) postoperative CRP, ( D ) postoperative IL-1β, and ( E ) postoperative IL-6 in all 93 cases of patients after abdominal surgery.

Article Snippet: CRP (catalog no. SEKH-0138) and Klotho (catalog no. SEKH-0280) ELISA kits were purchased from Solarbio (Beijing, China), while IL-1β (catalog no. DLB50) and IL-6 (catalog no. D6050B) ELISA kits were obtained from R&D Systems (Minneapolis).

Techniques:

Journal: Journal of Inflammation Research

Article Title: The Relationship Between Decreased Serum Klotho Protein and Cognitive Impairment in Patients After Abdominal Surgery

doi: 10.2147/JIR.S593405

Figure Lengend Snippet: Proposed mechanism linking decreased postoperative serum Klotho levels to postoperative cognitive dysfunction (POCD) after abdominal surgery. Surgery and prolonged anesthesia lead to a reduction in serum Klotho levels, which contributes to increased oxidative stress and systemic inflammation, as indicated by elevated levels of CRP, IL-1β, and IL-6. These processes promote neuroinflammation and synaptic dysfunction, resulting in cognitive decline and POCD. Arrows indicate the direction of the effects, illustrating how decreased Klotho mediates the relationship between perioperative stress, inflammation, and postoperative cognitive impairment.

Article Snippet: CRP (catalog no. SEKH-0138) and Klotho (catalog no. SEKH-0280) ELISA kits were purchased from Solarbio (Beijing, China), while IL-1β (catalog no. DLB50) and IL-6 (catalog no. D6050B) ELISA kits were obtained from R&D Systems (Minneapolis).

Techniques:

Journal: Communications Medicine

Article Title: Repurposing caspofungin as a small-molecule inhibitor of Clostridium perfringens α-toxin for treatment of gas gangrene

doi: 10.1038/s43856-026-01503-y

Figure Lengend Snippet: A HUVECs were cultured for 4 h in the presence or absence of 500 ng/ml α-toxin and the indicated concentrations of micafungin or caspofungin. Cell viability was measured using Cell Counting Kit-8 and expressed relative to the control. B HUVECs were cultured in the presence or absence of 500 ng/ml α-toxin and 50 µM micafungin or caspofungin, and a LDH leakage assay was performed using Cytotoxicity LDH Assay Kit-WST according to the manufacturer’s protocol. LDH release was expressed relative to the control. C HUVECs were cultured for 24 h in the presence or absence of 10 ng/ml α-toxin, 10 µg/ml peptidoglycan (PGN), and the indicated concentrations of micafungin or caspofungin. Interleukin-6 (IL-6) levels in the culture medium were measured using a human IL-6 Quantikine ELISA kit according to the manufacturer’s protocol. D Bone marrow cells were cultured for 3 h in the presence or absence of 100 ng/ml α-toxin and the indicated concentrations of micafungin or caspofungin, and the cells were labeled with antibodies diluted in PBS containing 2% FBS after blocking Fc-receptors with purified rat anti-mouse CD16/CD32. To quantify the amount of CD11b, a flow cytometry analysis was performed using Guava easyCyte. The mean fluorescence intensities of CD11b in Gr-1 + cells are shown. E C57BL/6J mice were injected intraperitoneally with 600 ng of α-toxin and 300 µg of micafungin or caspofungin, which had been pre-mixed and diluted in PBS. The survival of mice was monitored, and Kaplan–Meier survival curves are shown. The total number of mice used in the experiments was 63. Median survival (hours): Control, 6; caspofungin, not reached; micafungin, 6. A one-way ANOVA ( A – D ) or the Log-rank test ( E ) was employed to assess significance. Values are the mean ± standard deviation for panels ( A – D ) ( n = 3).

Article Snippet: After the treatment of cells with α-toxin, peptidoglycan (PGN), and the test compounds, culture supernatants were harvested and interleukin-6 levels were measured using a human IL-6 Quantikine ELISA kit (R&D Systems, MN, USA).

Techniques: Cell Culture, Cell Counting, Control, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, Labeling, Blocking Assay, Purification, Flow Cytometry, Fluorescence, Injection, Standard Deviation